Consumables for Organoids and Cell Lines
| Item | Vendor | Part Number | Unit Size | Cost |
| Media | ||||
| DPBS; no calcium, no magnesium | ThermoFisher | 14190250 | 10 x 500mL | $120 |
| DMEM; high glucose, no glutamine | ThermoFisher | 11960069 | 10 x 500mL | $140 |
| Advanced DMEM/F12 | ThermoFisher | 12634028 | 10 x 500mL | $600 |
| Fetal Bovine Serum (FBS); Premium Select, Heat Inactivated | Bio-Techne | S11550H | 500mL bottle | $300 |
| TrypLE Express | ThermoFisher | 12605-010 | 100mL bottle | $15 |
| Recovery Cell Culture Freezing Medium | ThermoFisher | 12648010 | 50mL bottle | $120 |
| Matrigel | Corning | CB356239 | 5 x 10pk | $350 |
| Recombinant Human R-spondin-1 | STEMCELL | 78213.3 | 1000ug | $2,500 |
| Plastic Consumables | ||||
| 6-well plates, tissue culture treated | Fisher Scientific | 07-200-80 | cs/100 | $140 |
| 12-well plates, tissue culture treated | Fisher Scientific | 07-200-81 | cs/100 | $170 |
| Corning Cryogenic Vials with closures, 2mL | Fisher Scientific | 337421 | cs/500 | $190 |
| Cell Strainers, 100 um | Fisher Scientific | 08-771-19 | cs/50 | $75 |
| Cell Strainers, 70 um | Fisher Scientific | 08-771-20 | cs/50 | $75 |
| 1000uL filter tips | USA Scientific | 1122-1730 | cs/10 | $80 |
| 200uL filter tips | USA Scientific | 1120-8710 | cs/10 | $70 |
| 20uL filter tips | USA Scientific | 1123-1710 | cs/10 | $70 |
| BD Tuberculin Syringes; 1mL | Fisher Scientific | 14-823-434 | 8 x 200pk | $380 |
| Needle, 25G x 5/8in, 100/bx, 10bx/case | Fisher Scientific | 14826AA | case | $180 |
| 50mL conical tubes | Fisher Scientific | 05-538-60 | cs/500 | $135 |
| 15mL conical tubes | Fisher Scientific | 05-538-59b | cs/500 | $120 |
| Ultra Spin Microcentrifuge Tubes; 1.5mL | DOT Scientific | 711-FTG | 500/pk | $15 |
| Microcentrifuge Tubes; 0.6mL | LabSource | T52-436 | 5000/cs | $350 |
| 5mL serological pipettes | Fisher Scientific | 13-675-22 | 200/cs | $45 |
| 10mL serological pipettes | Fisher Scientific | 14-955-234 | 200/cs | $45 |
| 25mL serological pipettes | Fisher Scientific | 07-200-575 | 200/cs | $95 |
Cell-line and Organoid Services
| Service | Description | Unit | Cost | |
| Fresh Biopsies | Biopsies collected and stored in Transport Media for future organoid generation | 4-6 biopsies per region collected | $7.50 | |
| Fresh surgical tissue | Surgical tissue resections stored in Transport Media or formalin | 5-10g per region collected | $11.25 | |
| Fresh Blood | Blood collected from clinical patients (bulk, plasma, serum, etc.) | per tube | $3.75 | |
| Fresh mucosal brush | Mucosal brush collected during colonoscopy | per brush, per region | $9.50 | |
| Fresh luminal aspirate | Aspirate samples collected in clinic | per sample, per region | $5.15 | |
| Frozen Biopsies in RNAlater | Biopsies collected and stored in RNAlater for metatranscriptomics | 4-6 biopsies per region collected | $10 | |
| Conditioned media containing WRN | Conditioned media for organoid work | 10 mL | $20.50 | |
| Conditioned media containing Noggin | Conditioned media for organoid work | 10 mL | $20.50 | |
| Conditioned media containing R-spondin | Conditioned media for organoid work | 10 mL | $20.50 | |
| Mouse enteroid expansion media | growth media for organoids | 50 mL | $110.50 | |
| Mouse colonoid expansion media | growth media for organoids | 50 mL | $110.50 | |
| Human organoid expansion media | growth media for organoids | 50 mL | $250.10 | |
| Frozen cell lines | Vials of WRN, Noggin, and R-spondin secreting cell lines | 1 cryovial | $127 | |
| Glycerol stocks of bacterial strains | Bacterial strains generated from human or mouse microbiomes | 1 cryovial | $115 | |
| Frozen Mouse organoid line | Frozen vial of mouse enteroid | 1 cryovial | $190.45 | |
| Frozen Human organoid line | Frozen vial of human organoid line | 1 cryovial | $207.50 | |
| Generation of fresh organoid line | Making new lines from freshly collected tissue sample | 2 wells fresh, or 1 vial frozen | $622.50 | |
| Organoid experiment | Generating organoids, expanding them, and running experiments with them | 4-6 wells fresh, per treatment/condition | $1500+ | *This is a variable fee depending on what the collaborator needs us to. Base minimum will be $1500 |
| Active enteroid or colonoid line (mouse) | Aliquot of mouse line currently in active culture | 2 wells of 6-well plate | $450.00 | |
| Active organoid line (human) | Aliquot of human line currently in active culture | 2 wells of 6-well plate | $450.00 | |
| Consulting services | Hourly rate | per hour | $110 | |
| Teaching for growth of organoids | Hourly rate | per hour | $110 | |
| Technician fee/labor | Hourly rate | per hour | $55 |