Consumables for Organoids and Cell Lines
| Item | Vendor | Part Number | Unit Size | Cost |
| Media | ||||
| DPBS; no calcium, no magnesium | ThermoFisher | 14190250 | 10 x 500mL | $120 |
| DMEM; high glucose, no glutamine | ThermoFisher | 11960069 | 10 x 500mL | $140 |
| Advanced DMEM/F12 | ThermoFisher | 12634028 | 10 x 500mL | $600 |
| Fetal Bovine Serum (FBS); Premium Select, Heat Inactivated | Bio-Techne | S11550H | 500mL bottle | $300 |
| TrypLE Express | ThermoFisher | 12605-010 | 100mL bottle | $15 |
| Recovery Cell Culture Freezing Medium | ThermoFisher | 12648010 | 50mL bottle | $120 |
| Matrigel | Corning | CB356239 | 5 x 10pk | $350 |
| Recombinant Human R-spondin-1 | STEMCELL | 78213.3 | 1000ug | $2,500 |
| Plastic Consumables | ||||
| 6-well plates, tissue culture treated | Fisher Scientific | 07-200-80 | cs/100 | $140 |
| 12-well plates, tissue culture treated | Fisher Scientific | 07-200-81 | cs/100 | $170 |
| Corning Cryogenic Vials with closures, 2mL | Fisher Scientific | 337421 | cs/500 | $190 |
| Cell Strainers, 100 um | Fisher Scientific | 08-771-19 | cs/50 | $75 |
| Cell Strainers, 70 um | Fisher Scientific | 08-771-20 | cs/50 | $75 |
| 1000uL filter tips | USA Scientific | 1122-1730 | cs/10 | $80 |
| 200uL filter tips | USA Scientific | 1120-8710 | cs/10 | $70 |
| 20uL filter tips | USA Scientific | 1123-1710 | cs/10 | $70 |
| BD Tuberculin Syringes; 1mL | Fisher Scientific | 14-823-434 | 8 x 200pk | $380 |
| Needle, 25G x 5/8in, 100/bx, 10bx/case | Fisher Scientific | 14826AA | case | $180 |
| 50mL conical tubes | Fisher Scientific | 05-538-60 | cs/500 | $135 |
| 15mL conical tubes | Fisher Scientific | 05-538-59b | cs/500 | $120 |
| Ultra Spin Microcentrifuge Tubes; 1.5mL | DOT Scientific | 711-FTG | 500/pk | $15 |
| Microcentrifuge Tubes; 0.6mL | LabSource | T52-436 | 5000/cs | $350 |
| 5mL serological pipettes | Fisher Scientific | 13-675-22 | 200/cs | $45 |
| 10mL serological pipettes | Fisher Scientific | 14-955-234 | 200/cs | $45 |
| 25mL serological pipettes | Fisher Scientific | 07-200-575 | 200/cs | $95 |
Cell-line and Organoid Services
| Product/Service Description | Per Unit | Internal UChicago Users & External Affiliated Users | External Academic/Non-Profit Users | External Commercial Users |
| Fresh Biopsies | SAMPLE | $6.51 | $8.99 | $8.99 |
| Fresh Surgical Tissue | SAMPLE | $14.40 | $19.87 | $19.87 |
| Fresh Blood | TUBE | $6.26 | $8.64 | $8.64 |
| Fresh Mucosal Brush | SAMPLE | $9.38 | $12.95 | $12.95 |
| Fresh Luminal Aspirate | SAMPLE | $8.04 | $11.10 | $11.10 |
| Frozen Biopsies in RNAlater | SAMPLE | $9.27 | $12.79 | $12.79 |
| Conditioned Media Containing WRN | UNIT | $61.64 | $85.07 | $85.07 |
| Conditiioned Media Contiaining Noggin | UNIT | $42.75 | $59.00 | $59.00 |
| Conditioned Media Containing R-spondin | UNIT | $42.75 | $59.00 | $59.00 |
| Mouse Enteroid Expansion Media | UNIT | $133.48 | $184.21 | $184.21 |
| Mouse Colonoid Expansion Media | UNIT | $133.48 | $184.21 | $184.21 |
| Human Organoid Expansion Media | UNIT | $239.29 | $330.22 | $330.22 |
| Frozen Cell Lines | VIAL | $124.05 | $171.19 | $171.19 |
| Glycerol Stocks of Bacterial Strains | VIAL | $115.91 | $159.96 | $159.96 |
| Frozen Mouse Organoid Line | VIAL | $188.67 | $260.36 | $260.36 |
| Frozen Human Organoid Line | VIAL | $211.24 | $291.51 | $291.51 |
| Generation of Fresh Organoid Line | VIAL | $674.90 | $931.36 | $931.36 |
| Organoid Experiment | PLATE | $1,631.40 | $2,251.33 | $2,251.33 |
| Active Enteroid or Colonoid Line (Mouse) | PLATE | $398.20 | $549.52 | $549.52 |
| Active Organoid Line (Human) | PLATE | $453.98 | $626.49 | $626.49 |
| Consulting Services | HOUR | $99.82 | $137.75 | $137.75 |
| Teaching for Growth of Organoids | HOUR | $108.02 | $149.07 | $149.07 |
| Technician fee/Labor | HOUR | $67.02 | $92.49 | $92.49 |
| Mass Spectrometry | RUN | $116.46 | $160.71 | $160.71 |
| Murine Colonoscopy Experiments | HOUR | $155.10 | $214.04 | $214.04 |